The pro-inflammatory phenotype of human macrophages is restricted by the activity of the orphan nuclear receptor Nurr1

Abstract The nuclear receptor related 1 protein (Nurr1) is a member of the orphan hormone family whose expression has been associated with several inflammatory diseases. Macrophages are key regulators processes, yet information about role Nurr1 in human macrophages limited. In present study we examined and activity steady state activated monocyte-derived macrophages. Pro- anti-inflammatory were derived vitro by culture blood monocytes hematopoietic cytokines GM-CSF M-CSF, respectively. was predominant pro-inflammatory phenotype. Accordingly, its induced variety stimuli such as TLR-2, -3 -4 ligands, TNF, IFN-β, IL-4. Pro-inflammatory exposed to agonist C-DIM12 decreased their production IL-1β, IL-6, reactive oxygen species. Conversely, deficient produced enhanced levels IL-6. Mechanistically, agonists partially abrogated activation NF-κB signaling pathway LPS, rendering cells lower rate p65 translocation. These results suggest that linked phenotype where it may constitute brake attenuate synthesis mediators. Supported grants from Secretaria de Educación Pública-Consejo Nacional Ciencia y Tecnología (SEP-CONACYT, #A1-S-9430)